But, some of the variable regions of the humanized antibodies are derived from mouse DNA. The antigenic region of the humanized antibodies is derived from human DNA. The monoclonal antibody lines are expressed in the animal cell cultures.įigure 2: Monoclonal Antibodies (brown: human, blue: non-human)īottom row: humanized, chimeric/humanized, human Recombinant DNA technology combines the human and mouse DNA. This reduces the risk of rejection by the human immune system when administrated. Generally, two-thirds of the human DNA sequence is involved in the production of a chimeric antibody. – Comparison of Key Differences Key TermsĬhimeric Antibody, Chimerization, Humanized Antibody, Humanization, Therapeutic PurposesĪ chimeric antibody is a combination of proteins made from human DNA and mouse DNA.
What is the Difference Between Chimeric and Humanized Antibody What are the Similarities Between Chimeric and Humanized AntibodyĤ. They are used for the administration of humans as anticancer drugs. The main difference between chimeric and humanized antibody is that the chimeric antibody is a monoclonal antibody with a large stretch of non-human proteins whereas the humanized antibody is a non-human antibody with a protein sequence that has been modified to increase the similarity to human antibodies. Chimeric antibodies track down and illuminize the tumor regions while the antigenic region of the humanized antibodies is derived from human DNA and the variable region is derived from mouse DNA.Ĭhimeric and humanized antibody are two types of monoclonal antibodies produced by mice or rodents under laboratory conditions.